Abstract

Most species of Megastigmus are considered important economic pests that grow in seeds, especially of conifers. Accurate identification of species is a crucial step for the biological research of parasitic pests and the further application of biological control. However, their large variety, small size, similar morphology and different growth and development stages have brought great challenges to taxonomic research. Traditional morphological identification often takes a long time and this requires us to seek a new method for rapid and accurate identification. Therefore, the better identification of Megastigmus urgently needs to be combined with molecular methods to help taxonomic development.
Here, Megastigmus daduheensis sp. n. (Chalcidoidea: Megastigmidae) was identified, based on morphology and molecular markers, such as COI and Cytb. M. daduheensis sp. n. is distinct from other known species of the same genus in the morphology. The results of the molecular phylogenetic tree, similarity alignment and genetic distance indicate that the COI and Cytb sequences of M. daduheensis sp. n. are highly similar to M. sobinae and M. duclouxiana, but there are some genetic differences. The genetic distances of M. daduheensis sp. nov. with M. duclouxiana and M. sabinae were 0.34 and 0.33 and the percentages of shared base pairs were 76.3% and 76.8%, respectively. Both morphological and molecular data classified M. daduheensis sp. n. as a new species. The obtained COI and Cytb sequences of M. daduheensis sp. n. can be used as DNA barcodes, providing molecular data for rapid and accurate identification of this species in the future.

Addeddate

2024-09-09 16:16:23

Bhl_virtual_titleid

210877

Bhl_virtual_volume

v.11 (2023)

Call number

10_3897_BDJ_11_e102828

Call-number

10_3897_BDJ_11_e102828

Foldoutcount

0

Genre

article

Identifier

newspeciesmegas11chen

Identifier-ark

ark:/13960/s286tg4h4xj

Identifier-bib

10_3897_BDJ_11_e102828

Identifier-doi

10.3897/BDJ.11.e102828

Identifier-elocator

e102828

Ocr

tesseract 5.3.0-6-g76ae

Ocr_detected_lang

en

Ocr_detected_lang_conf

1.0000

Ocr_detected_script

Latin

Ocr_detected_script_conf

1.0000

Ocr_module_version

0.0.21

Ocr_parameters

-l eng

Page_number_confidence

79

Page_number_module_version

1.0.5

Page_range

1-16

Pages

16

Pdf_degraded

invalid-jp2-headers

Pdf_module_version

0.0.25

Possible copyright status

In copyright. Digitized with the permission of the rights holder.

Ppi

300

Source

Biodiversity Data Journal 11

Year

2023

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